Our homocysteine elisa is designed for detection and quantitation of homocysteine in plasma, serum, lysates, or biological fluid. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate dna, proteins, lipids, and other intracellular metabolites. The maxdiscovery homocysteine enzymatic assay kit has the capacity for 48 determinations or testing of 24 samples in duplicate assuming 12 wells for standards. Elevated concentration of homocysteine is a frequently observed finding in the blood of these patients. Total homocysteine biochemical assay kit intented use the formosa biomedical technology total homocysteine biochemical assay kit is an in vitro assay for the quantitative determination of total homocysteine in plasma. Maxdiscovery homocysteine enzymatic assay kit specs. Do not use the kit beyond the expiration date opened kit coated assay plate may be stored for up to 1 month at 2 8c. Food and drug administration fda has granted diazyme 510k clearance to market the enzymatic homocysteine assay kit. Homocysteine elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Homocysteine is a sulfurcontaining amino acid and a risk factor involved. Elisa kit for homocysteine hcy ced984ge panspecies. Hcy elisa kit is a sandwich enzyme immunoassay for the quantitative measurement of hcy homocysteic acid. About diazyme diazyme laboratories is a division of general atomics. The homocysteine hplc assay kit is for research use only and should not be used for diagnostic procedures.
Elevated levels of homocysteine can indicate a risk for numerous diseases, including vascular and heart disease. Research article lcystathionine protects against homocysteine induced mitochondriadependent apoptosis of vascular endothelial cells xiuli wang,1,2 yi wang,1 lulu zhang,1 da zhang,1 lu bai,1 wei kong,3 yaqian huang,1 chaoshu tang,3,4 junbao du,1 and hongfang jin 1 1department of pediatrics, peking university first hospital, beijing 34, china 2research unit of clinical diagnosis and. Functions of analyte homocysteine hcyh is a sulphur. Our homocysteine elisa is designed for detection and quantitation of homocysteine in plasma, serum. Pdf biochemistry of homocysteine in health and diseases. Is it a clinically important cardiovascular risk factor. Pdf the amino acid homocysteine hcy, formed from methionine has profound importance in health and. Biovisions homocysteine elisa kit is a competitive elisa assay for the quantitative measurement of homocysteine in human serum, plasma and cell culture supernatants.
Total homocysteine is defined as the sum of all homocysteine species in plasmaserum, including free and proteinbound forms. The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteineselective. Principle of the assay the microtiter plate provided in this kit has been precoated with an antibody specific to homocysteine. Protein bound homocysteine is reduced to free homocysteine and enzymatically converted to sadenosyllhomocysteine sah in a separate procedure prior to the immunoassay 14. Caymans dna methylation elisa kit is a competitive assay that can be used for the quantification of 5methyl2deoxycytidine in urine, culture supernatants, plasma, and other sample matrices. The eagle biosciences sadenosyllhomocysteine sah elisa assay kit is intended for the quantitative determination of sadenosyllhomocysteine sah in serum or plasma. If dont make recent use, better keep it store at 20c. Liquid stable 2part homocysteine assay proven utility first promoted for cardiac risk assessment,homocysteine now appears to be an accurate frontline indicator for a wide variety of diseases throughout a persons life. Enzymatic homocysteine 3 reagent diazyme laboratories. Lsf10591 it is important that you read this entire manual carefully before starting your experiment. Patients who are taking methotrexate, carbamazepine, phenytoin. High homocysteine levels prevent via h2 s the cocl2 induced alteration of lymphocyte viability.
The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Folate supplementation has escalated over recent years with the knowledge of its many benefits. Application lhomocysteine has been used as a supplement in cell culture media. Nebivolol prevents the increase of asymmetric dimethylarginine and oxidants in hyperhomocysteinemic rats mustafa ahmet huyut, et al,international journal of the cardiovascular academy,2019. Total homocysteine thcy represents the sum of all forms of hcy including forms of oxidized, protein bound and free. Pdf quantitation of the homocysteine content in wine. Research article lcystathionine protects against homocysteineinduced mitochondriadependent apoptosis of vascular endothelial cells xiuli wang,1,2 yi wang,1 lulu zhang,1 da zhang,1 lu bai,1 wei kong,3 yaqian huang,1 chaoshu tang,3,4 junbao du,1 and hongfang jin 1 1department of pediatrics, peking university first hospital, beijing 34, china 2research unit of clinical diagnosis and. Homocysteine is an amino acid intermediate that is synthesized from methionine via sadenosylmethionine sam and sadenosylhomocysteine sah. Although they may lack some of the vitamins involved in the metabolism of homocysteine, the increased levels of total homocysteine are mainly due to impaired removal of homocysteine from the blood by the kidney. The eagle biosciences sadenosyllhomocysteine sah elisa assay kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures. Buy hcy elisa kit, human homocysteine hcy elisa kitp39442. This assay is a competitive elisa where samples and an antihomocysteine antibody are added to a. The diazyme homocysteine enzymatic assay kit is intended for the in vitro quantitative determination of total lhomocysteine in serum or plasma. Improved hplc method for total plasma homocysteine detection and quantification.
The assay can assist in diagnosis and treatment of patients suspected of having hyperhomocysteinemia and homocystinuria. Myonectin ctrp15 is a novel nutrientresponsive myokine secreted by skeletal muscle to regulate wholebody fatty acid metabolism. Linearity lq homocysteine, calibration verification. As one of the b vitamins, folate, or vitamin b9, is involved in many bodily functions and deficiency can cause disease in not only the elderly, but infants too. Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. Introduction homocysteine is an amino acid, which is produced out of methionine. Simultaneous detection and screening of t 833 c and g 919 a mutations of the. Cell biolabs homocysteine elisa kit is a competitive enzyme immunoassay developed for the detection and quantitation of homocysteine in plasma, serum, lysates, or other biological fluid samples. Pdf improved hplc method for total plasma homocysteine. Homocysteine plasma, urine, dried blood spots 1 name and description of analyte 1.
Biovisions homocysteine assay kit allows for quantification of total homocysteine in biological fluids such as plasma and serum. The shelf life is 12 months when the kit is properly stored. The metabolism and significance of homocysteine in nutrition and. Biochemphysiol actions elevated homocysteine levels in plasma and tissue are associated with brain atrophy in alzheimer. Bioassay technology laboratory bt lab is a brand of shanghai korain biotech co ltd specialized in lab supplies of elisa kit, antibodies, protein for life science research. Folic acid decreases astrocyte apoptosis by preventing oxidative stressinduced telomere attrition li w,international journal of molecular sciences,2019.
The eagle biosciences homocysteine hplc assay kit is intended for the quantitative determination of homocysteine in plasma and serum. Homocysteine hcy is a sulfur containing amino acid formed during the metabolism of. Homocysteine assay kit fluorometric k531 biovision, inc. This kit is not approved for use in humans or for clinical diagnosis. Introduction over 1 million of americans died of cardiovascular disease cvd such as heart attack or stoke. Homocysteine hplc assay eagle biosciences elisa assay kit. Although such patients lack some of the vitamins involved in the metabolism of homocysteine, the elevated homocysteine levels are mainly due to impaired. Homocysteine elisa kit alpha diagnostic international. The density of color is proportional to the amount of homocysteine captured from the samples. It is a competitive eia assay which can detect homocysteine down to 98.
Homocysteine elisa kit lsf25097 is a 96well enzymelinked immunosorbent assay for the quantitative detection of all species homocysteine in plasma, serum. Homocysteine is a sulfurcontaining amino acid that plays a crucial role in methylation reactions. The laboratory test on the girls blood sample confirmed hhcy 33. Enzymatic homocysteine 3 reagent 2020 diazyme laboratories, inc. These immunoassays are extremely flexible and have adequate sensitivity to go down to the drug levels found in most forensic matrices. Alternatively, hcy can combine with serine to form cystathionine. Erythroferrone myonectin ctrp15 fam2b human elisa kit. Pdf elevated plasma homocysteine is associated with an increased risk of myocardial infarction, stroke, and venous thromboembolism. Download our elisa brochure pdf enzyme linked immunosorbent assay elisa screening techniques are widely utilized by toxicologists to screen forensic specimens for drugs of abuse.
Small volumes of homocysteine elisa kit vials may occasionally. Homocysteine linearity is assayed calibration verification material consisting of five levels of human based serum. Try to keep it in a sealed aluminum foil bag, and avoid the damp. The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteineselective enzyme, generating an intermediate product.
The kit has a detection sensitivity limit of 10 ngml homocysteinebsa. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly. This immunoassay kit allows for the in vitro quantitative determination of general homocysteine concentrations in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Correlation of tsh with homocysteine and lipid profile in hypothyroidism a malik, et al,j liaquat uni med health sci,2018. Lcystathionine protects against homocysteineinduced. Pdf recent clinical research has pointed at hyperhomocysteinemia as an independent risk factor in a number of. Effect of seabuckthorn seed oil in reducing cardiovascular risk factors. The stability of kit is determined by the loss rate of activity. Methionine elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples.
We aimed to investigate the serum concentrations of vitamin b12. The enzyme is specific for the lform of homocysteine, which is the only. The presence of elevated total homocysteine levels in plasma or serum has been connected to the increased risk of thrombosis, neural tube defects and atherosclerotic vascular disease. The diazyme homocysteine enzymatic a ssay kit is intended for the in vitro quantitative determination of total lhomocysteine in serum or plasma. For the quantitative determination of endogenic human homocysteine hcy. To meet every single need of the customer we aim to provide high quality at best price and service with our professional staff. Creative diagnostics provides homocysteine antibodies and elisa kits for science research use, feel free to contact us. Ctrp15 also is a erythroid factor on ironregulation. The report will summarize current or previous lots. A longitudinal controlled trial on hypertensive subjects. Rat homocysteine assay kit fluorescence mouse homocysteine assay kit.
Maxdiscovery homocysteine enzymatic assay kit bioo. Pdf comparison of serum b12, folate and homocysteine. Homocysteine assay kit fluorometric ab228559 allows for quantification of total homocysteine in biological fluids such as plasma and serum. Homocysteine a strong risk factor for cardiovascular disease. We cannot guarantee the sample manual posted online is the most current manual. Homocysteine homeostasis and betainehomocysteine s. All species homocysteine elisa kit sandwich elisa user manual catalog no. Management of cardiovascular disease medical policies were merged and entitled.
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